Healthians

 Microbial assay of antibiotics as per IP. INTRODUCTION The microbiological assay of an antibiotic is based upon a comparison of the inhibition of growth of micro-organisms by measured concentrations of the Free website hits antibiotics under examination with that produced by known concentrations of a standard preparation of the antibiotic having a known activity. Gernally two types of method are mentioned in pharmacopeias books (IP,BP,USP)                       The cylinder-plate method (Method A) depends upon diffusion of the antibiotic from a vertical cylinder through a solidified agar layer in a Petri dish or plate to an extent such that growth of the added micro-organism is prevented entirely in a zone around the cylinder containing a solution of the antibiotic. The turbidimetric method (Method B) depends upon the inhibition of growth of a microbial culture in a uniform solution of the antibiotic in a flu...